Laboratory Exercises in Microbiology
by Harley, John P.Edition: 7th
Format: Paperback
Pub. Date: 2007-02-05
Publisher(s): McGraw-Hill Science/Engineering/Math
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Summary
Laboratory Exercises in Microbiology, 7/e has been prepared to accompanyPrescott, Harley and Klein's Microbiology, 7e, written by new authors Joanne Willey, Linda Sherwood, and Christopher Woolverton. Like the text, the laboratory manual provides a balanced introduction to laboratory techniques and principles that are important in each area of microbiology.
Table of Contents
| Microscopic Techniques | |
| Bright-Field Light Microscope and Microscopic Measurement of Organisms | |
| The Hanging Drop Slide and Bacterial Motility | |
| Dark-Field Light Microscope | |
| Phase-Contrast Light Microscope | |
| Flourescence Microscope | |
| Bacterial Morphology and Staining | |
| Negative Staining | |
| Smear Preparation and Simple Staining | |
| Gram Stain | |
| Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures | |
| Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin) | |
| Capsule Staining | |
| Flagella Staining: West and Difco's SpotTest Methods | |
| Basic Laboratory and Culture Techniques | |
| Microbiological Culture Media Preparation and Sterilization | |
| Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures | |
| Spread-Plate Technique | |
| The Streak-Plate Technique and Different Media | |
| Pour-Plate Technique | |
| Cultivation of Anaerobic Bacteria | |
| Determination of Bacterial Numbers | |
| Biochemical Activities of Bacteria | |
| Carbohydrates I: Fermentation and B-Galactosidase Activity | |
| Carbohydrates II: Triple Sugar Iron Agar Tests | |
| Carbohydrates III: Starch Hydrolysis | |
| Lipids: Lipid Hydrolysis | |
| Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility | |
| Proteins, Amino Acids, and Enzymes II: The IMViC Tests | |
| Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis | |
| Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis | |
| Proteins, Amino Acids, and Enzymes V: Catalase Activity | |
| Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity | |
| Proteins, Amino Acids, and Enzymes VII: Oxidase Test | |
| Proteins, Amino Acids, and Enzymes VIII: Urease Activity | |
| Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Test | |
| Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination | |
| Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction | |
| Rapid Multitest Systems | |
| The API 20E System | |
| The Enterotube II System | |
| Unknown Identification | |
| Using the First Edition of Bergey's Manual of Systematic Bacteriology to Identify Bacteria | |
| General Unknown | |
| Environmental Factors Affecting Growth of Microorganisms | |
| Temperature | |
| pH | |
| Osmotic Pressure | |
| The Effects of Chemical Agents on Bacteria I: Disinfectants | |
| The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method) | |
| Hand Washing, Environmental Sampling, and Microbiological Monitoring | |
| Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods | |
| Environmental and Food Microbiology | |
| Standard Coliform Most Probably Number (MPN)Test and Presence-Absence Coliform Test | |
| Membrane Filter Technique for Coliform and Fecal Streptococci; KONFIRM Test for Fecal Coliforms | |
| Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers | |
| Enumeration of Soil Microorganisms | |
| Bacterial Count of a Food Product | |
| Examination of Milk for Bacteria | |
| Medical Microbiology | |
| Agglutination Reactions: Blood Groups | |
| Isolation of Normal Microbiota from the Human Body | |
| Staphylococci | |
| Pneumococci | |
| Streptococci | |
| Neisseriae | |
| Aerobic and Anaerobic Endospore-Forming Bacteria | |
| Survey of Selected Eucaryotic Microorganisms | |
| Fungi I: Yeasts | |
| Fungi II: Phycomycetes, Ascomycetes, and Basidiomycetes | |
| Microbial Genetics and Genomics | |
| Bacterial Mutation | |
| Bacterial Transformation | |
| Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids | |
| Isolation of Genomic DNA from Saccharomyces cerevisiae | |
| Isolation and Purification of Genomic DNA from Escherichia coli | |
| Identifying Archaea and Bacteria Using the Internet and Computer-Assisted Gene Analysis | |
| Dilutions with Sample Problems | |
| Metric and English Measurement Equivalents | |
| Transmission-Absorbance Ta | |
| Table of Contents provided by Publisher. All Rights Reserved. |
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