The book provides a systematic and comprehensive look at recent advances in the field, detailing the methodologies and strategies behind these approaches. This provides the reader with the distinctive advantages and disadvantages of the presenten methodologies and strategies in a target focused process and allows the reader to adapt and implement the strategies for new applications. The first three parts of the book are dedicated to the directed evolution, rational design and semi-rational design of proteins while the last two parts present applications in industrial and medical biotechnology.

Dr. Huimin Zhao is the Steven L. Miller Chair of chemical and biomolecular engineering, and professor of chemistry, biochemistry, biophysics, and bioengineering at the University of Illinois at Urbana-Champaign (UIUC). He received his B.S. degree in Biology from the University of Science and Technology of China in 1992 and his Ph.D. degree in Chemistry from the California Institute of Technology in 1998 under the guidance of Dr. Frances Arnold. Prior to joining UIUC in 2000, he was a project leader at the Industrial Biotechnology Laboratory of the Dow Chemical Company. He was promoted to full professor in 2008. Dr. Zhao served as a consultant for over 10 companies such as Pfizer, Maxygen, BP, Gevo, and zuChem, and a Scientific Advisory Board member of Gevo, Myriant Technologies, Toulouse White Biotechnology (TWB) and AgriMetis. He was a member of National Academies' study group on Industrialization of Biology: A Roadmap to Accelerate Advanced Manufacturing of Chemicals. Dr. Zhao has authored and co-authored over 260 research articles and over 20 issued and pending patent applications with several being licensed by industry. In addition, he has given plenary, keynote or invited lectures in over 290 international meetings, universities, industries, and research institutes. His primary research interests are in the development and applications of synthetic biology tools to address society's most daunting challenges in health, energy, and sustainability, and in the fundamental aspects of enzyme catalysis, cell metabolism, and gene regulation.

Introduction
Editor
Contributors

Section I. Directed Evolution
I-1. Directed evolution of proteins
I-2. MALDI MS imaging tools
I-3. Cell surface display
I-4. PACE
I-5. In vivo biosensors
I-6. Continuous protein evolution

Section II. Rational Design
II-1. Computational protein design
II-2. Predicting protein functions using computational tools
II-3. Enzyme engineering by QM/MM simulations
II-4. Structural modeling

Section III. Semi-rational Design
III-1. Iterative saturation mutagenesis
III-2. ProSAR
III-3. Data-driven protein engineering

Sections IV. Applications in industrial technology
IV-1. Artificial metalloenzyme engineering
IV-2. P450 engineering
IV-3. Engineering Proteins using non-natural amino acids
IV-4. Protein engineered biomaterials

Section V. Applications in medical biotechnology
V-1. Antibody engineering
V-2. CAR-T immunotherapy engineering
V-3. Development of genome editing tools
V-4. Non-antibody scaffold engineering
V-5. Molecular tools for cellular imaging